MRPL12 抗体
Applications
| In-house applications |
ELISA, WB, IHC, IF, FC, IP Application not tested? Please see our Guarantee. |
| Published applications |
WB |
Publications |
3 citations –view here |
| Species specificity |
Human,Mouse,Rat; other species not tested. |
| Published Species |
Human |
|
Images
Prev12Next
 HeLa cells were subjected to SDS PAGE followed by western blot with 14795-1-AP(MRPL12 antibody) at dilution of 1:500 Immunohistochemical of paraffin-embedded human colon cancer using 14795-1-AP(MRPL12 antibody) at dilution of 1:100 (under 40x lens) Immunofluorescent analysis of Hela cells, using MRPL12 antibody 14795-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). IP Result of anti-MRPL12 (IP:14795-1-AP, 4ug; Detection:14795-1-AP 1:500) with HeLa cells lysate 2000ug. 1X10^6 HeLa cells were stained with 0.2ug MRPL12 antibody (14795-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
|
| Positive WB detected in |
HeLa cells, COLO 320 cells, MCF7 cells, mouse skeletal muscle tissue |
| Positive IP detected in |
HeLa cells |
| Positive IHC detected in |
Human colon cancer tissue |
| Positive IF detected in |
Hela cells |
| Positive FC detected in |
HeLa cells |
| Recommended dilution |
WB : 1:500-1:5000
IP : 1:200-1:2000
IHC : 1:20-1:200
IF : 1:20-1:200 |
MRPL12 抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
| Source |
Rabbit |
Purification method |
Antigen affinity purification |
| Isotype |
IgG |
Storage |
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen |
MRPL12 fusion protein ag6504 |
Full name |
Mitochondrial ribosomal protein L12 |
Calculated molecular
weight |
21 kDa |
Observed molecular
weight |
21 kDa |
GenBank accession
number |
BC002344 |
Gene ID (NCBI) |
6182 |
| Gene symbol |
MRPL12 |
Synonyms |
5c5 2, FLJ60124, L12mt, MRP L12, MRP L31/34, MRPL12, MRPL7, MRPL7/L12, RPML12 |
Background MRPL12 抗体
|
Mammalian MRPL12 was the first mitochondrial ribosomal protein to be characterized, and is encoded by a nuclear gene. The protein forms a homodimer, localizes predominantly to mitochondria and binds to the large mitochondrial ribosomal subunit [PMID:862670]. It is required for CycD/Cdk4-induced cell growth. In addition, forming a ribosomal stalk with MRPL7, MRPL12 is required for the recruitment of translation elongation factors Tu and G to the large ribosomal subunit [PMID:12370014]. It also a part of a novel regulatory mechanism that coordinates mitochondrial transcription with translation and/or ribosome biogenesis during human mitochondrial gene expression. The antibody reacts specifically with 21kDa MRPL12 protein. MRPL12 抗体
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