MDH1抗体
Applications
| In-house applications |
ELISA, WB, IF, FC, IP, IHC Application not tested? Please see our Guarantee. |
| Published applications |
WB |
Publications |
3 citations –view here |
| Species specificity |
Human,Mouse,Rat; other species not tested. |
| Published Species |
Human |
|
Images
Prev12Next
 HL-60 cells were subjected to SDS PAGE followed by western blot with 15904-1-AP(MDH1 antibody) at dilution of 1:500 Immunofluorescent analysis of HepG2 cells, using MDH1 antibody 15904-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). IP Result of anti-MDH1 (IP:15904-1-AP, 4ug; Detection:15904-1-AP 1:1000) with mouse liver tissue lysate 6400ug. Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using 15904-1-AP( MDH1 Antibody) at dilution of 1:100 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9). Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using 15904-1-AP( MDH1 Antibody) at dilution of 1:100 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9). 1X10^6 HepG2 cells were stained with 0.2ug MDH1 antibody (15904-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
|
| Positive WB detected in |
HL-60 cells, HepG2 cells, human kidney tissue, human spleen tissue, mouse liver tissue |
| Positive IP detected in |
Mouse liver tissue |
| Positive IHC detected in |
Human renal cell carcinoma tissue, human liver cancer tissue |
| Positive IF detected in |
HepG2 cells |
| Positive FC detected in |
HepG2 cells |
| Recommended dilution |
WB : 1:500-1:5000
IP : 1:500-1:5000
IHC : 1:50-1:500
IF : 1:10-1:100 |
MDH1抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
| Source |
Rabbit |
Purification method |
Antigen affinity purification |
| Isotype |
IgG |
Storage |
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen |
MDH1 fusion protein ag8744 |
Full name |
Malate dehydrogenase 1, NAD (soluble) |
Calculated molecular
weight |
334aa,36 kDa |
Observed molecular
weight |
36 kDa |
GenBank accession
number |
BC001484 |
Gene ID (NCBI) |
4190 |
| Gene symbol |
MDH1 |
Synonyms |
Cytosolic malate dehydrogenase, MDH s, MDH1, MDHA, MOR2 |
Background MDH1抗体
|
MDH1(Malate dehydrogenase, cytoplasmic) is also named as MDHA and belongs to the LDH/MDH superfamily and MDH type 2 family which catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. It can exsit as a dimer and the dimeric MDH1 is the mitochondrial isoenzyme, whereas the tetrameric MDH2 is the glycosomal isoenzyme.(PMID:10693743). MDH1抗体
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