葡聚糖硫酸钠(Dextran Sulfate Sodium，DSS)是葡聚糖的聚阴离子衍生物，喂食果蝇会破坏肠上皮中肠道表皮细胞之间的连接从而造成肠道损伤，引起多条通路应答调控干细胞的自我更新及分化，补充受损细胞，维持肠道稳态。  

图1. 果蝇肠道干细胞分裂分化示意图

实验动物

5-10日龄果蝇，雌性 

实验材料

DSS（yeasen DSS 60316ES,MW36000-50000） 

实验步骤

1. 用5%的蔗糖溶液配制喂食培养基；
2. 对照组：5%蔗糖培养基（SC），实验组：3% DSS（培养基配制）；
3. 预先用500 μL的喂食培养基将2.5cm×3.75cm的层析纸润湿，然后放入空的培养瓶中；
4. 果蝇放入含有层析纸的瓶中，29℃培养三天，期间每天将存活的果蝇移入含有新鲜培养基的空瓶中；
5. 培养三天后，切开中肠，用GFP（绿色）和PH3（红色）抗体和DRAQ5（蓝色）进行免疫染色。

实验结果

图2.  DSS诱导果蝇中ISC前体细胞增殖^[1]

 通过免疫染色中不同的标记方法，量化了肠道干细胞（ISC），ISC前体细胞发生增值，表明DSS诱导果蝇模型肠道发生损伤。

翌圣DSS产品优势

翌圣生物提供高品质DSS（Cat.NO：60316ES，MW:36000~50000），具有以下优势：

1. 高纯度（＞98%），硫含量17-19%，游离硫＜0.2%，大量数据文献支持，广泛应用于UC模型的构建。

2. 多种属动物中均可造模：小鼠、大鼠、斑马鱼、猪、果蝇；

3. 症状表现与人UC极度相似：可用于研究急、慢性结肠炎的发生发展机制，也可用于药物的药效研究。

4. 简单易行，成模率高，重复性强

5. 联合氧化偶氮甲烷 (azoxymethane, AOM)用药，可用于诱发结肠炎相关性癌症 (colitis associated cancer, CAC) 动物模型，成功模拟IBD诱发CAC的过程。

建模效率与进口产品一致，产品价格低至进口价格的1/3，大量现货库存。

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2022

[1] Lujuan Xing, Lijuan Fu, Songmin Cao,et al.The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice[J]. Nutrients 2022, 14, 1479. IF=5.717

[2] Lei-NingChen,TaoJing,Zi-BinLin,et al.Metabolomic and transcriptomic responses of mouse testis to the dextran sulfate sodium induced colitis[J].Reproductive Toxicology.2022, Pages 35-42.IF=3.143

[3] YuangengLi,PingYu,WenwenFu,et al.Polysaccharides from Panax ginseng C. A. Meyer alleviated DSS-induced IBD by inhibiting JAK2/STAT1/NLPR3 inflammasome signalling pathway in mice[J].Journal of Functional Foods.2022, 105013. IF=4.451

[4] Wang S,  Huang J,  Tan KS, et al.Isosteviol Sodium Ameliorates Dextran Sodium Sulfate-Induced Chronic Colitis through the Regulation of Metabolic Profiling, Macrophage Polarization, and NF-B Pathway[J].Oxidative Medicine and Cellular Longevity. 2022,4636618. IF=5.076

[5] Chenglu Sun, Jingpeng Hao,Hong Qin,et al.Endometrial Regenerative Cell-Derived Conditioned Medium Alleviates Experimental Colitis.Stem Cells Int 2022;2022 IF=3.869

2021

[1] Li Zhao, Fei Wang , Zhengwei Cai，et al.Improving drug utilization platform with injectable mucoadhesive hydrogel for treating ulcerative colitis[J].Chemical Engineering Journal.424(2021)130464. IF=16.744

[2] Hao H,  Zhang X,  Tong L,  Liu Q,et al.Lactobacillus plantarumEffect of Extracellular Vesicles Derived From  Q7 on Gut Microbiota and Ulcerative Colitis in Mice[J].Frontiers in Immunology.2021.777147 .IF=7.561

[3] Lingjun Tong, Xinyi Zhang , Haining Hao,et al.Lactobacillus rhamnosus GG Derived Extracellular Vesicles Modulate Gut Microbiota and Attenuate Inflammatory in DSS-Induced Colitis Mice[J].Nutrients.2021, 13, 3319. IF=5.717

[4] Zhipeng Gu , Yujie Zhu, Fengfeng Mei,et al.Tilapia head glycolipids protect mice against dextran sulfate sodium-induced colitis by ameliorating the gut barrier and suppressing NF-kappa B signaling pathway[J].International Immunopharmacology.96 (2021) 107802. IF=3.943

[5] JialiDong,YuanLi,HuiwenXiao,et al.Oral microbiota affects the efficacy and prognosis of radiotherapy for colorectal cancer in mouse models[J].Cell reports.2021, 109886.IF=9.423

[6] Yaohua Fan,Yanqun Fan,Kunfeng Liu,et al.Edible Bird’s Nest Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57BL/6J Mice by Restoring the Th17/Treg Cell Balance[J].Frontiers in Pharmacology.2021.632602.IF=7.561

2020

[1] Jia-Rong Huang, Sheng-Te Wang, Meng-Ning Wei,et al.Piperlongumine Alleviates Mouse Colitis and Colitis-Associated Colorectal Cancer[J].Frontiers in Pharmacology.2020.586885. IF=7.561

[2] Gao X, Fan W, Tan L, et al. Soy isoflavones ameliorate experimental colitis by targeting ERα/NLRP3 inflammasome pathways[J]. The Journal of Nutritional Biochemistry, 2020, 83.IF=6.048

[3] Li, Y., Dong, J., Xiao, H., Zhang, S., Wang, B., Cui, M., & Fan, S. Gut commensal derived-valeric acid protects against radiation injuries. Gut Microbes,.2020 .1–18.IF=10.245

BEFORE 2020

[1] Oehlers SH, Flores MV, Hall CJ, Crosier KE, Crosier PS. Retinoic acid suppresses intestinal mucus production and exacerbates experimental enterocolitis. Dis Model Mech. 2012 Jul;5(4):457-67.IF=4.973

[2] Kim CJ, Kovacs-Nolan JA, Yang C, Archbold T, Fan MZ, Mine Y. l-Tryptophan exhibits therapeutic function in a porcine model of dextran sodium sulfate (DSS)-induced colitis. J Nutr Biochem . 2010 Jun;21(6):468-75.IF=6.048

[3] Karpowicz , P., Perez, J. & Perrimon , N.,. The Hippo tumor suppressor pathway regulates intestinal stem cell regeneration. Development (Cambridge, England), 2010,137(24), pp.4135–4145.IF=6.868

[4] Fan H, Chen W, Zhu J, et al. Toosendanin alleviates dextran sulfate sodium-induced colitis by inhibiting M1 macrophage polarization and regulating NLRP3 inflflammasome and Nrf2/HO-1 signaling[J]. International immunopharmacology, 2019, 76: 105909.IF=3.943

往期精彩

1.不同分子量葡聚糖硫酸钠选购指南：高品质DSS葡聚糖硫酸钠盐

2.DSS结肠炎造模整体解决方案：Dextran Sulfate Sodium Salt（DSS） 溃疡性结肠炎模型的建立 (yeasen.com) 

参考文献

[1] Fangfang Ren,et al.PNAS.2010.107(49) 21064-21069

产品中心

· 分子生物学

· 蛋白研究

· 重组蛋白

· 抗体

· 细胞培养与分析

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· 生命科学研究

· 高通量测序

· 分子诊断

· 生物制药

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